Fish breeding by using hormones is gaining popularity now days. The hormone secreted from the pituitary gland of the fish is used for fish breeding. The glands are collected from the head of the fish. After killing the adult fish, the brain is removed first and the gland is taken out. These glands are then put in absolute alcohol. They should not be warm. The hormones are injected during rainy season. The atmospheric temperature should be 30 degree Celsius and there should be drizzling rain, while injecting the hormones. The preserved glands are transferred into a white paper and dried nicely. After that it has to be put in a tissue homogenizer and ground well. Into this ground glands, add distilled water and dissolve the hormone. If we pour this mixture into a centrifuge, the solution comes to the top, and this is used for injecting the fishes. While injecting the hormone to the male and female fish, it should be in a prescribed quantity. For a female fish weighing 1 kg, the hormone should be taken from glands weighing up to 10 gm. injecting such hormones increase the drive to mate and reproduce among the fish. The maximum quantity of hormone solution injected to a fish should not exceed 1.0 milliliter.
While giving such injections, the male fish tends to have lesser weight and so while injecting the ratio should be for 1 female fish, 2 male fishes. The weight of 2 male fishes will be equal to that of a female house. The fishes are drawn using nets and kept in rubber cushions for giving injections. The hormone is injected between the dorsal fin and the tail of the fish. The injected fishes are kept under water using happas build using bamboo poles. ‘Happa’ is a chamber built with cloth in between bamboo poles. The upper portion of the happa can be opened and closed. The fishes mate and lay eggs within 6 hours. If the fishes do not mate the injection should be given again.